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| Registros recuperados : 352 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
14/03/2003 |
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Data da última atualização: |
14/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - A |
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Autoria: |
MELO, N. F. de. |
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Afiliação: |
NATONIEL FRANKLIN DE MELO, CPATSA. |
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Título: |
Somatic embryogenesis and ploidy stability in cassava (Manihot esculenta Crantz) cultivars regenerated by in vitro culture of young leaves. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Cytologia, Tpkyo, v. 67, n. 4, p. 337-341, 2002. |
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Idioma: |
Inglês |
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Conteúdo: |
The regeneration and cytogenetic analysis of 7 cassava (Manihot esculenta Crantz) cultivars obtained by organogenesis and somatic embryogenesis were investigated. In the first phase, callus formation was induced in young leaves using MS medium supplemented with 1.0, 5.0, 10.0, 15.0 and 20.0 mg/l of 2,4-D or without a growth regulator (control). In the second phase (development), the calli obtained in the above treatments were transferred to MS medium supplemented with factorial combinations of GA3 and BAP at 0, 0.5, 1.0, 1.5 and 2.0 mg/l. Results of the first phase showed that 2,4-D induced the formation of both friable and compact calli. The compact calli displayed root and shoot formation, whilst somatic embryos were obtained only from friable calli in the medium with 1.0 mg/l GA3. Plants obtained from somatic embryos were regenerated in MS medium supplemented with 0.04 mg/l NAA and 0.05 mg/l GA3. Cytogenetic analysis in 167 regenerated plants from somatic embryogenesis and 176 from organogenesis showed 2n= 36 as a normal diploid chromosome number. |
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Thesagro: |
Análise; Calo; Citogenética Vegetal; Embriogénese; Mandioca; Manihot Esculenta; Micropropagação; Variedade. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/176744/1/Separata-9620.pdf
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Marc: |
LEADER 01711naa a2200217 a 4500
001 1137733
005 2018-05-14
008 2002 bl uuuu u00u1 u #d
100 1 $aMELO, N. F. de
245 $aSomatic embryogenesis and ploidy stability in cassava (Manihot esculenta Crantz) cultivars regenerated by in vitro culture of young leaves.
260 $c2002
520 $aThe regeneration and cytogenetic analysis of 7 cassava (Manihot esculenta Crantz) cultivars obtained by organogenesis and somatic embryogenesis were investigated. In the first phase, callus formation was induced in young leaves using MS medium supplemented with 1.0, 5.0, 10.0, 15.0 and 20.0 mg/l of 2,4-D or without a growth regulator (control). In the second phase (development), the calli obtained in the above treatments were transferred to MS medium supplemented with factorial combinations of GA3 and BAP at 0, 0.5, 1.0, 1.5 and 2.0 mg/l. Results of the first phase showed that 2,4-D induced the formation of both friable and compact calli. The compact calli displayed root and shoot formation, whilst somatic embryos were obtained only from friable calli in the medium with 1.0 mg/l GA3. Plants obtained from somatic embryos were regenerated in MS medium supplemented with 0.04 mg/l NAA and 0.05 mg/l GA3. Cytogenetic analysis in 167 regenerated plants from somatic embryogenesis and 176 from organogenesis showed 2n= 36 as a normal diploid chromosome number.
650 $aAnálise
650 $aCalo
650 $aCitogenética Vegetal
650 $aEmbriogénese
650 $aMandioca
650 $aManihot Esculenta
650 $aMicropropagação
650 $aVariedade
773 $tCytologia, Tpkyo$gv. 67, n. 4, p. 337-341, 2002.
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Embrapa Semiárido (CPATSA) |
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